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Catalog Number | orb1529558 |
---|---|
Category | Antibodies |
Description | TPR Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 2313 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:25 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | TPR |
UniProt ID | P12270 |
NCBI | NP_003283.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | tumor potentiating region; translocated promoter r Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human TPR by western blot.Samples: Whole cell lysate from HeLa, LNCaP, HEK293T, Hep-G2, and Jurkat cells. Antibody: Affinity purified rabbit anti-TPR antibody orb1529558 at 0.04 µg/ml.
Detection of mouse TPR by immunohistochemistry. Sample: FFPE section of mouse liver. Antibody: Affinity purified rabbit anti-TPR used at a dilution of 1:100 (2µg/ml).
Detection of human TPR by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TPR used at a dilution of 1:200 (1µg/ml).
Detection of human TPR by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-TPR antibody.
IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |