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Rabbit anti-Toca-1 Antibody
Catalog Number: orb1524774
| Catalog Number | orb1524774 |
|---|---|
| Category | Antibodies |
| Description | Toca-1 Antibody |
| Target | Toca-1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 1000 µg/ml |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Immunogen | Between 450 and 500 |
| UniProt ID | Q5T0N5 |
| Tested applications | IHC, IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | formin-binding protein 1-like; TOCA1; toca-1; tran Read more... |
| Note | For research use only |
| NCBI | NP_001157945.1 |
Detection of human Toca-1 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Toca-1 antibody orb1524774 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Toca-1 was also immunoprecipitated by rabbit anti-Toca-1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human Toca-1 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Toca-1 (Cat. No. orb1524774) used at a dilution of 1: 1, 000 (1µg/ml) Detection: DAB
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