You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Rabbit anti-Pygo2 Antibody
Catalog Number: orb1526099
| Catalog Number | orb1526099 |
|---|---|
| Category | Antibodies |
| Description | Pygo2 Antibody |
| Target | Pygo2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 1 and 50 |
| UniProt ID | Q9BRQ0 |
| Tested applications | IHC, IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | 1190004M21Rik; pygopus 2; pygopus homolog 2 |
| Note | For research use only |
| NCBI | NP_612157.1 |
Detection of human and mouse Pygo2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pygo2 antibody orb1526099 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Pygo2 was also immunoprecipitated by rabbit anti-Pygo2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human Pygo2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Pygo2 (Cat. No. orb1526099) used at a dilution of 1: 200 (1µg/ml) Detection: DAB
- Item 1 of 2
- Item 1 of 2
- Item 1 of 2
