Rabbit anti-LOX-1 Recombinant Monoclonal Antibody

• Catalog Number: orb1710817
• Category: Antibodies
• Description: LOX-1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR262L
• Tested applications: ICC, IHC, WB
• Reactivity: Human
• Immunogen: Residues 61-273 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: LOX-1
• UniProt ID: P78380
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: LOX1; ox LDL receptor 1; SLOX1; scavenger receptor
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human LOX-1 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, MCF-7, and SiHa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human DLD-1 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human NCI-H226 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710817). Secondary: HRP-conjugated goat anti-rabbit IgG.