Rabbit anti-INSM1 Recombinant Monoclonal Antibody

• Catalog Number: orb1733232
• Category: Antibodies
• Description: Rabbit anti-INSM1 Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR272L
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 75 and 125
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: INSM1
• UniProt ID: Q01101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: zinc finger protein IA-1; insulinoma-associated pr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human INSM1 by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, NCI-H1155, HeLa, SHP-77, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse INSM1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, Beta-TC-6, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of human pancreas. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunohistochemistry. Sample: FFPE section of neuroendocrine tumor. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human INSM1 by immunocytochemistry. Sample: FFPE section of SHP-77 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse INSM1 by immunocytochemistry. Sample: FFPE section of Beta-TC-6 cells. Antibody: Rabbit anti-INSM1 recombinant monoclonal antibody (orb1733232). Secondary: HRP-conjugated goat anti-rabbit IgG.