Rabbit anti-HMGN1 Antibody

• Catalog Number: orb1526759
• Category: Antibodies
• Description: HMGN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 50 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 5 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:1,000 - 1:5,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HMGN1
• UniProt ID: P05114
• NCBI: NP_004956.5
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: nonhistone chromosomal protein HMG-14; high-mobili
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HMGN1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HMGN1 antibody orb1526759 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. HMGN1 was also immunoprecipitated by rabbit anti-HMGN1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minute (A) and 30 seconds (B).

Detection of human and mouse HMGN1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-HMGN1 (Cat. No. orb1526759) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human HMGN1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-HMGN1 (Cat. No. orb1526759) used at a dilution of 1: 2, 000 (0.5µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100 (5µg/ml).