Rabbit anti-HJURP Antibody

• Catalog Number: orb1525930
• Category: Antibodies
• Description: HJURP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 675 and 725
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HJURP
• UniProt ID: Q8NCD3
• NCBI: NP_060880.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Holliday junction recognition protein; up-regulate
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HJURP by western blot. Samples: Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody (orb1525930) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human HJURP by western blot of immunoprecipitates. Samples: Whole cell lysate (500 µg for IP; 2% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HJURP antibody (orb1525930) used for IP at 6 µg per reaction. HJURP was also immunoprecipitated by a previous lot of this antibody (orb1525930) and rabbit anti-HJURP antibody.

Detection of human HJURP by immunohistochemistry.Sample: FFPE section of human lung carcinoma.Antibody: Affinity purified rabbit anti-HJURP (Cat. No. orb1525930) used at a dilution of 1: 5, 000 (0.2µg/ml) Detection: DAB