Rabbit anti-BRD9 Recombinant Monoclonal Antibody

• Catalog Number: orb1519613
• Category: Antibodies
• Description: BRD9 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR153J
• Tested applications: FC, ICC, IHC, IP, SW-Size, WB
• Reactivity: Human, Mouse
• Immunogen: Between 547 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BRD9
• UniProt ID: Q9H8M2
• NCBI: NP_076413.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: bromodomain-containing protein 9; LAVS3040; PRO985
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse BRD9 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (orb1519613) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human BRD9 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (orb1519613) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human BRD9 by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (orb1519613) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Detection of mouse BRD9 by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (orb1519613) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.