PYY Antibody

• Catalog Number: orb1264317
• Category: Antibodies
• Description: PYY Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PYY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the C-terminal region of human PYY.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: PYY
• UniProt ID: P10082
• NCBI: P10082
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Peptide YY, PYY, PYY-I, PYY
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PYY Antibody in MCF-7 cell line lysates (35 ug/lane)

PYY Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of PYY Antibody with MCF-7 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.