PYROXD1 Antibody

• Catalog Number: orb1248528
• Category: Antibodies
• Description: PYROXD1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: TCAEQLATHFPSED
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55+48kDa bands observed in Human Colorectal Cancer lysates (calculated MW of 55.8kDa according to NP_079130.2). The observed 48kDa band corresponds to earlier findings with different antibodies from other commercial sources and likely represents a breakdown product. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 55 + 48kDa
• Target: PYROXD1
• UniProt ID: Q8WU10
• NCBI: NP_079130.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PYROXD1, pyridine nucleotide-disulphide oxidoreduc
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55+48kDa bands observed in Human Colorectal Cancer lysates (calculated MW of 55.8kDa according to NP_079130.2). The observed 48kDa band corresponds to earlier findings with different antibodies from other commercial sources and likely represents a breakdown product. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248528 (1 ug/ml) staining of Human Colorectal Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248528 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.