PSMB3 Antibody

SKU: orb1249052

Description

PSMB3 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 24kDa band observed in lysates of cell line K562 (calculated MW of 22.9kDa according to NP_002786.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NLYELKEGRQ
• Target: PSMB3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

PSMB3, proteasome (prosome, macropain) subunit, beta type, 3, HC10-II, MGC4147, proteasome beta 3 subunit, proteasome chain 13, proteasome component C10-II, proteasome theta chain

orb1249052 (1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249052 (10 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PSMB3

UniProt

P49720

RefSeq (Protein):NP_002786.2