PROX-1-S514 Antibody

• Catalog Number: orb1265087
• Category: Antibodies
• Description: PROX-1-S514 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from human PROX1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 83 kDa
• Target: PROX1
• UniProt ID: Q92786
• NCBI: Q92786
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Prospero homeobox protein 1, Homeobox prospero-lik
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: SW620 whole cell lysate Lysates/proteins at 20 ug per lane.

PROX-1-S514 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PROX-1-S514 Antibody for immunohistochemistry.