Protein G Biotin Conjugated

• Catalog Number: orb348762
• Category: Proteins
• Description: Protein G Biotin Conjugated
• Tested applications: ELISA, IHC, WB
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
• Form/Appearance: Lyophilized
• Purity: Protein G Biotin Conjugated was prepared from chromatographically pure recombinant Protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Protein G. No reaction was observed against anti-Protein A.
• Conjugation: Biotin
• Hazard Information: Non-Toxic
• UniProt ID: P19909
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: ProG, Streptococcus G protein, Protein G Biotin Co
• Note: For research use only
• Application notes: Protein G Biotin Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype.
• Expiration Date: 6 months from date of receipt.

Motors containing inactive subunits exhibit slower start times, lower packaging speeds, and more pausing. a Schematic of the optical trapping assay to measure the packaging dynamics of a single T4 motor. A multi-stream laminar flow chamber (left) is used to manipulate samples and buffer. A bead coated with anti-T4 antibodies is captured in one trap in a flow stream containing ATP (green stream; step ①). Another bead conjugated with arrested T4 capsid–gp17-DNA motors is captured in a second optical trap in the stream containing non-hydrolyzable analog ATP-γS (red stream; step ②). The motor bound to DNA in the presence of ATP-γS cannot initiate packaging due to a lack of energy source. To form a tether between the bead pair, the beads are moved close for 1 s and separated apart to detect binding of capsid to antibody (step ③). After a constant force (F = 5 pN) load is applied to the tether, the traps are moved into ATP and packaging is detected as the decrease of DNA extension when DNA is translocated into the capsid by the motor (step ④). b Extension of unpackaged DNA vs. time, under constant force load. Packaging activity is observed within seconds of entering the ATP stream. Representative packaging trajectories with WT (blue) and ISD motors (orange) are shown. c Distribution of the start times of WT (blue, n = 44) and ISD motors (orange, n = 62), defined as the dwell time between entry into the ATP stream and the start of packaging. d Distribution of mean pause-free packaging velocity derived from each packaging trace. ISD motors (orange) show a lower packaging velocity than WT motors (blue). e Histogram of the logarithm of pause frequency, defined as the number of pauses per kb packaged. ISD motors (orange) exhibit more pauses than WT motors (blue) on average. Motors with long start times (black stars; c–e) have a lower packaging velocity and higher pause frequency. The number of trajectories used is indicated by n = #.

Signal response of Nobuto serum samples from 112 canids profiled with the F1-LPA. (A) Detection of F1 immunoreactivity was accomplished using a genus-specific secondary anti-IgG antibody. (B) Detection of F1 immunoreactivity was accomplished using biotinylated conjugates of staphylococcal protein A (p/n orb348752), streptococcal protein G (p/n orb348762), and staphylococcal protein A and streptococcal protein G (A/G), with sera diluted 1:20. The dashed line indicates the threshold for positive and negative samples using an S/N ratio of 10.

Single molecule fluorescence measurements of refilled heads. Quantification of packaging by single molecule fluorescence assay. (A and C) Fluorescence images of immobilized T4 heads packaged with Cy3 (83-bp) and Cy5 (39-bp) DNAs, respectively. One-fourth of the 70 µm ×35 µm imaging area is shown in each case. (B and D) Histograms showing the number of heads packaged with Cy3 or Cy5 DNAs. The number of heads showing fluorescence in more than 30 images was averaged in each case.