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Catalog Number | orb1152481 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Protein C |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | HPC-4 |
Tested applications | Blocking, ELISA |
Reactivity | Human, Monkey |
Isotype | IgG1 |
Immunogen | This antibody was raised by immunising mice with human protein C. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | Protein C |
UniProt ID | P04070 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Autoprothrombin IIA; C-tag; Protein C tag; Protein Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ELISA using HPC-4 and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the anti-Protein C antibody HPC-4 (orb1152481; green line) and isotype control (orb256381, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 at a concentration of 5 ug/ml.
Immunofluorescence staining of HEK293 cells with Anti-Protein C HPC-4. Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Protein C expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of HPC-4 (orb1152481) (1:200 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
Western Blot using Anti-Protein C antibody HPC-4. Protein C expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric mouse version of HPC-4 (orb1152481) at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, IP, WB | |
Human, Mouse, Rabbit, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |