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Protein A

Catalog Number: orb82628

DispatchUsually dispatched within 2-3 weeks
$ 240.00
Catalog Numberorb82628
CategoryProteins
DescriptionProtein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgGbinding domains E-D-A-B-C aligned in series. It migrates with an apparent molecular mass of 45kDa in SDS-PAGE.
TargetProtein A. The interaction between Protein A and IgG is not equivalent for all species. Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.
ConjugationUnconjugated
ConcentrationA280 of 0.1% solution: -0.165
Buffer/PreservativesLyophilized with no additive. Dissolve in distilled water or saline.
Purity> 95% pure by SDS-PAGE analyses.
MW45 kDa
Tested applicationsFA, HPLC, SDS-PAGE
Biological OriginE.coli
Biological ActivityLess than 0.1 EU/μg of Protein A as determined by LAL method.
StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
NoteFor research use only
Protein A

SDS-PAGE analysis of Protein A

  • tdTomato Antibody [orb182397]

    ELISA,  IF,  IHC-Fr,  IHC-P,  WB

    Other

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • ATP1A1 Rabbit Polyclonal Antibody [orb1816802]

    IF,  IHC-Fr,  IHC-P

    Mouse, Rat

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Sodium Potassium ATPase Mouse Monoclonal Antibody [orb704302]

    IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 50 μl
  • Anti-SF3B3 Antibody [orb1402085]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Sodium Potassium ATPase Recombinant Rabbit Monoclonal Antibody [orb559158]

    IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Human, Mouse, Rat

    Rabbit

    Recombinant

    Unconjugated

    25 μl, 100 μl, 50 μl