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Product Overview
Product Name PPP2R5E antibody
Catalog Number orb18322
Species/HostGoat
ReactivityBovine, Canine, Human, Mouse, Rat
Predicted Reactivity Bovine, Canine
ConjugationUnconjugated
Tested applicationsELISA, WB
Immunogen C-LKRGLRRDGIIPT
Target PPP2R5E
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 54.7 kDa
Uniprot ID Q16537
NCBI NP_006237.1
Entrez 5529
Product Description

Goat polyclonal antibody to PPP2R5E

Application Notes
Dilution Range ELISA: 1:16000, WB: 0.1-0.3 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.

WB: Approx 55kDa and 40kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 μg/ml. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).
Validation Images
orb18322 staining (0.5ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18322 staining (0.5ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of  Human Cerebellum (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using PPP2R5E antibody
Western blot analysis of Human Cerebellum (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using PPP2R5E antibody
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