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Catalog Number | orb18322 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PPP2R5E |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml |
Conjugation | Unconjugated |
MW | 54.7 |
Target | PPP2R5E |
Entrez | 5529 |
Protein Sequence | LKRGLRRDGIIPT |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PPP2R5E antibody, anti protein phosphatase 2, Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa and 40kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 μg/ml. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9). |
Expiration Date | 12 months from date of receipt. |
orb18322 staining (0.5ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western blot analysis of Human Cerebellum (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using PPP2R5E antibody
ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish | |
Canine, Equine, Guinea pig, Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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