PPP2R1B Mouse Monoclonal Antibody

• Catalog Number: orb783399
• Category: Antibodies
• Description: PPP2R1B Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: H6T2
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1,Κ
• Immunogen: Recombinant human PPP2R1B.
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:25, IHC-F=1:25, ICC/IF=1:25, IF=1:25, Flow-Cyt=1:25
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: PPP2R1B
• UniProt ID: P30154
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 2AAB_HUMAN; beta isoform of regulatory subunit A,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Jurkat. Primary Antibody (green line): Mouse Anti-PPP2R1B antibody (orb783399), dilution: 1:25, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: 1:400, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human spleen sections), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPP2R1B) Monoclonal Antibody, Unconjugated (orb783399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Jurkat cell lysates, Lane 2: Human brain tissue lysates, Lane 3: Mouse brain tissue lysates, Lane 4: Mouse lung tissue lysates, Lane 5: Rat brain tissue lysates, Primary: Anti-PPP2R1B (orb783399) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 60 kD.

U-2OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPP2R1B) monoclonal Antibody, Unconjugated (orb783399) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, Alexa Fluor® 555 conjugated with Phalloidin (red) was used to stain the cell Cytoplasmic actin.DAPI (blue) was used to stain the cell nuclei.