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Catalog Number | orb749746 |
---|---|
Category | Antibodies |
Description | Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 4F10 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | Mouse IgM, kappa |
Immunogen | A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human Podocalyxin was used as the immunogen for the PODXL antibody. |
Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
Purity | PEG precipitation |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This PODXL antibody is available for research use only. |
Entrez | 5420 |
UniProt ID | O00592 |
Storage | Store the PODXL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the PODXL antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
FACS, IF, IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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