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Catalog Number | orb345448 |
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Category | Antibodies |
Description | PNK antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Primate |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein. |
Concentration | 1.46 mg/mL |
Dilution range | ELISA: 1:3,000 - 1:12,000, WB: 1:300 - 1:1,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human PNK protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Expect cross-reactivity with PNK from dog, mouse and rat sources, as only two amino acid residues change within the immunogen sequence (83% positive by BLAST). Cross-reactivity with PNK homologues from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q96T60 |
NCBI | 31543419 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-PNKP antibody, rabbit anti PNK antibod Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to PNK by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Y190 yeast cell lysate using PNK antibody
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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