PLOD1 Antibody

• Catalog Number: orb1269457
• Category: Antibodies
• Description: PLOD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000-1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 84 kDa
• Target: PLOD1
• UniProt ID: Q02809
• NCBI: Q02809
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1,
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000-1:2000
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Overlay histogram showing U-87 MG cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (NA168821) ) at 1/400 dilution for 40 min at 37oC. Isotype control antibody (blue line) was mouse IgG1 (1 ug/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Antibody staining PLOD1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:1000-1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: K562 whole cell lysates Lane 3: U-2OS whole cell lysates Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of lysates from A431, Hela, K562, MCF-7, U-87 MG cell line (from left to right), using PLOD1 Antibody at 1:1000 at each lane.

Western blot analysis in U251 cell line lysates (35 ug/lane).