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Catalog Number | orb259607 |
---|---|
Category | Antibodies |
Description | Plectin Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Plectin (2644-2671aa RFIEQEKAKLEQLFQDEVAKAQQLREEQ), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 531791 MW |
UniProt ID | Q15149 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Plectin;PCN;PLTN;Hemidesmosomal protein 1;HD1;Plec Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Plectin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Plectin using anti-Plectin antibody.Lane 1:human HeLa cell.
IF analysis of Plectin using anti-Plectin antibody. Plectin was detected in an immunocytochemical section of U20S cells.
IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of human intestine cancer tissue.
IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of human lung cancer tissue.
IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of mouse intestine tissue.
IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of rat intestine tissue.
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