PLC gamma 1 (phospho-Y1253) antibody

• Catalog Number: orb214403
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PLCG1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1253 of human PLC gamma 1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PLCG1
• Entrez: 5335, 25738, 18803
• UniProt ID: P10686, Q62077, P19174
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PLC1 antibody, anti 1-phosphatidylinositol 4,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PLC gamma 1 (Phospho-Y1253) expression in MCF7 EGF-treated (A), A431 (B), mouse brain (C), mouse liver (D), H9C2 EGF-treated (E) whole cell lysates. (Predicted band size: 148 kD; Observed band size: 155 kD)

Immunofluorescent analysis of PLC gamma 1 (Phospho-Y1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).