PLAUR Mouse Monoclonal Antibody

• Catalog Number: orb1184692
• Category: Antibodies
• Description: PLAUR Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6C7
• Tested applications: ELISA, FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Rat
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant human PLAUR protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 31 kDa
• Target: PLAUR
• UniProt ID: Q03405
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Plasminogen activator, urokinase receptor; Urokina
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A431. Primary Antibody (green line): Mouse Anti-PLAUR antibody (orb1184692), Dilution: 10 ul, Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Mouse IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLAUR) Monoclonal Antibody, Unconjugated (ascites of orb1184692) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLAUR) Monoclonal Antibody, Unconjugated (ascites of orb1184692) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLAUR) Monoclonal Antibody, Unconjugated (ascites of orb1184692) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.