| Category | Primary antibodies |
|---|---|
| Catalog Number | orb98431 |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | IHC-Fr, IP, WB |
| Reactivity | Canine, Human, Porcine |
| Clone ID | 12B11F8 |
| Isotype | IgG2a |
| Form/Appearance | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Conjugation | Unconjugated |
| UniProt ID | Q9Y446 |
| Application notes | 12B11F8 is suitable for immunocytochemistry (methanol-fixed cells), immunohistochemistry on frozen tissues, immunoprecipitation and Western blot detection. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 15 min. prior to incubation with primary antibody. Optimal antibody dilution should be determined by titRation; recommended range is 1:25-1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations. |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Description | Mouse monoclonal to Plakophilin-3 |
| Expiration Date | 12 months from date of receipt. |
| Note | For research use only. |
| Source | 12B11F8 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the extreme carboxyterminal amino acid residues 779-793 (KLHRDFRAKGYRKED) of human plakophilin-3 coupled to keyhole limpet hemocyanin |
- 3 images
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- Plakophilin 3 antibody [orb312777]
ELISA, ICC, IF, IHC
Bovine, Canine, Mouse, Porcine, Rat, Sheep
100 μl, 200 μl, 50 μl - Plakophilin 3 antibody (Biotin) [orb452285]
ELISA, ICC, IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Biotin
100 μl - 1 image
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- Plakophilin 3 antibody (HRP) [orb475790]
ELISA, ICC, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
HRP
100 μl
