PITX3 antibody

• Catalog Number: orb19943
• Category: Antibodies
• Description: Goat polyclonal antibody to PITX3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:1000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 31.8
• Target: PITX3
• Entrez: 5309
• Protein Sequence: PEHGCKGQEHSD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PITX3 antibody, anti paired-like homeodomain
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9;282(45):33192-200.; PMID: 17848564). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19943 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.