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Product Overview
Product Name PITX3 antibody
Catalog Number orb19943
Species/HostGoat
ReactivityHuman
ConjugationUnconjugated
Tested applicationsELISA, WB
Immunogen PEHGCKGQEHSD
Target PITX3
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 31.8 kDa
Uniprot ID O75364
NCBI NP_005020.1
Entrez 5309
Product Description

Goat polyclonal antibody to PITX3

Application Notes
Dilution Range ELISA: 1:1000, WB: 0.3-1 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.

WB: Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9;282(45):33192-200.; PMID: 17848564). Recommended concentration: 0.3-1 μg/ml.
Validation Images
orb19943 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19943 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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