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Product Overview
Product Name Pink n' Blue Protein Ladder
Catalog Number orb90560
Tested applicationsSDS-PAGE, WB
Hazard InformationNon-Toxic
Target Protein
Alternative Names
Product Properties
Form/Appearance Approximately 0.2-0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% (v/v) glycerol).
Storage Stable at 4°C for 3 months. Store at -20°C for 24 months.
Note For research use only.
Product Description

The Pink n' Blue Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The Pink n' Blue Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

Application Notes
Application Notes Experiment Notes:

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.

2. Mix thoroughly to ensure the solution is homogeneous. 3. Load the following volumes of the ladder on SDS-polyacrylamide gel

- 5 μl per well for mini-gels, 2.5 μl per well for blots

- 10 μl per well for large gels, 5 μl per well for blots

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