PIM2 antibody

• Catalog Number: orb131688
• Category: Antibodies
• Description: Goat polyclonal to PIM2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.5-1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 34.2
• Target: PIM2
• Entrez: 11040
• Protein Sequence: QTPAEDVPLNPSK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PIM2; pim-2 oncogene; pim-2h; proto-oncogene Pim-2
• Note: For research use only
• Application notes: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1 μg/ml.WB: In transfected HEK293 transiently expressing Human PIM2 (myc and DYKDDDDK tagged), a band of approx. 38kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human using PIM2 antibody

orb131688 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb131688 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb131688 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb131688 (1µg/ml) staining of HepG2 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.