Pig MIP1b ELISA Kit

• Catalog Number: orb1173618
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Beta(MIP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Beta(MIP1b) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Porcine
• Range: 78.13-5000 pg/mL
• Sensitivity: 30 pg/mL
• Protein Length: 3.5h
• Alternative names: MIP1 Beta, CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4,
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Expiration Date: Please enquire.