Phospho-MAPK11 (Thr180+Tyr182) Rabbit Polyclonal Antibody

• Catalog Number: orb4805
• Category: Antibodies
• Description: Phospho-MAPK11 (Thr180+Tyr182) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Equine, Gallus, Human, Porcine, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MAPK11 around the phosphorylation site of Thr180+Tyr182 EM(p-T)G(p-Y)VA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg /test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: MAPK11
• UniProt ID: Q15759
• RRID: AB_10929281
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: MAPK11(phospho Thr180/Tyr182); MAPK11(phospho T180
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-MAPK11 (Thr180+Tyr182)) Polyclonal Antibody, Unconjugated (orb4805) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.