PGK1 Antibody

• Catalog Number: orb1263474
• Category: Antibodies
• Description: PGK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:100For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: PGK1
• UniProt ID: P00558
• NCBI: P00558
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Phosphoglycerate kinase 1, Cell migration-inducing
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:100For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132) at 1:1000 at each lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of PGK1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.