PDPN antibody

• Catalog Number: orb20288
• Category: Antibodies
• Description: Goat polyclonal to PDPN
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:1000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 24.9; 24.7; 12.4; 12.2
• Target: PDPN
• Entrez: 10630
• Protein Sequence: EKVDGDTQTTVEKD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDPN antibody, anti podoplanin antibody, anti
• Note: For research use only
• Application notes: This antibody is expected to recognize all isoforms (NP_006465.3; NP_938203.2; NP_001006625.1; NP_001006626.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Experiments gave bands at approx 25kDa and 37kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. The 37kDa band corresponds to earlier findings in literature with different antibodies (Boucherot et al, Nephrol Dial Transplant. 2002 Jun;17(6):978-84; PMID: 12032185). This protein has a calculated MW of 24.9kDa according to NP_006465.3. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20288 (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.