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Catalog Number | orb63300 |
---|---|
Category | Tools |
Description | pCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 1141.14 g/mol (free acid); Detected MW: 1140.34 g/mol (free acid) |
SMILES | C(=C\C1=*(CC)c2c(C1(C)C)cc(S(=O)(=O)[O-])cc2)/C=C/C=C\1/N(c2c(C1(C)C)cc(cc2)S(=O)(=O)O)CCCCCC(=O)NCCCCCCOP(=O)(O[C@@H]1[C@@H](COP(=O)(O)O)O[C@@H](n2c(=O)nc(N)cc2)[C@@H]1O)O |
Formula | C48H66N6O18P2S2 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase.
Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |
Chemical structure of pCp-CY5 (Cy5)
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