PCBP2 monoclonal antibody (M07), clone 5F12

• Catalog Number: orb2292450
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant PCBP2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5F12
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: PCBP2 (AAH01155, 1 a.a. ~ 362 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMREESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLEEDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVKQICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGSDSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLTKLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWAGLDASAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMGSS
• NCBI: AAH01155
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to PCBP2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

PCBP2 monoclonal antibody (M07), clone 5F12 Western Blot analysis of PCBP2 expression in K-562.

Western Blot analysis of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody (M07), clone 5F12. Lane 1: PCBP2 transfected lysate (38.7 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody (M07), clone 5F12 (Cat # orb2292450). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (65.56 KDa).