PABPN1L Antibody

• Catalog Number: orb1269908
• Category: Antibodies
• Description: PABPN1L Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat, Xenopus
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PABPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human PABPN1L.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500-1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 30 kDa
• Target: PABPN1L
• UniProt ID: A6NDY0
• NCBI: A6NDY0
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Embryonic polyadenylate-binding protein 2, Embryon
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500-1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:500-1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 ug per lane.

PABPN1L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of PABPN1L Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.