P90RSK (Phospho-S363) antibody

• Catalog Number: orb540969
• Category: Antibodies
• Description: Rabbit polyclonal antibody to P90RSK (Phospho-S363)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: A synthetic phosphorylated peptide around S363 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
• Dilution range: WB: 1:500-1:2000, IP: 1:50-1:100
• Conjugation: Unconjugated
• MW: 90kDa
• UniProt ID: Q15418
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: HU-1, MAPKAPK1A, RSK, RSK1, p90Rsk, RPS6KA1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of various lysates using (orb540969) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.