p53 (phospho-Ser392) antibody

• Catalog Number: orb43788
• Category: Antibodies
• Description: Mouse monoclonal antibody to p53 (phospho-Ser392)
• Clonality: Monoclonal
• Clone Number: FP3.2 [FPS392]
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 μg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: p53 (pS392)
• Entrez: 7157
• UniProt ID: P04637
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti BCC7 antibody, anti FLJ92943 antibody, anti H
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 μg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). A - anti-p53 (total); B - anti-p53 (phospho Ser392). Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.

Western blotting analysis of phosphorylated human p53 using mouse monoclonal antibody FP3.2 (purified) in T47D cell line, which expresses p53. A) Phosphorylated p53 detected in T47D cell line. B) Absence of phosphorylated signal of p53 in dephosphorylated T47 cells (pretreated with calf intestinal alkaline phosphatase).