p53 Antibody

• Catalog Number: orb1336382
• Category: Antibodies
• Description: Mouse monoclonal antibody to p53 (TP53)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI3D5
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
• Antibody Type: Primary Antibody
• Dilution range: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Conjugation: Unconjugated
• MW: 43.5 kDa
• Target: p53
• UniProt ID: P04637
• NCBI: NM_000546
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)

Anti-TP53 mouse monoclonal antibody (TA502780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53)

HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502780), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (TA502780), (Red), compared to a nonspecific negative control antibody, (Blue).