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Product Overview
Product Name p53 antibody
Catalog Number orb11210
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Bovine, Equine, Porcine, Sheep
ConjugationUnconjugated
Tested applicationsELISA, ICC, IF, IHC-P, Flow-Cyt, WB
Immunogen KLH conjugated synthetic peptide derived between 261-300 amino acids of human p53
Target p53
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 43 kDa
Uniprot ID P04637 (Human); P02340 (Mouse); P10361 (Rat)
NCBI NP_000537.3; NM_000546.5
Entrez 7157
Product Description

Rabbit polyclonal antibody to p53

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, Flow-Cyt: 1μg/Test, ICC: 1:100-500, IF: 1:100-500
Validation Images
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using p53 antibody
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using p53 antibody
Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using p53 antibody
Immunohistochemical analysis of paraffin-embedded mouse endometrium tissue using p53 antibody
Immunohistrochemical analysis of formalin fixed and paraffin embedded human ovarian carcinoma using p53 antibody
Immunohistrochemical analysis of formalin fixed and paraffin embedded human ovarian carcinoma using p53 antibody
Western blot analysis of rat brain lysates(Lane1),human colon carcinoma lysates(Lane2) using p53 antibody
Western blot analysis of rat brain lysates(Lane1),human colon carcinoma lysates(Lane2) using p53 antibody
References

Wittmann, F. et al. To Be Or Not to Be: the "Smoker's Paradox" - An in-Vitro Study. Cell. Physiol. Biochem. 48, 1638-1651 (2018)

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