p-IKB alpha antibody

• Catalog Number: orb13487
• Category: Antibodies
• Description: p-IKB alpha antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32/36 (HD(p-S)GLD(p-S)M)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: phospho-IKB alpha (Ser32 + Ser36)
• UniProt ID: P25963
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: IKB alpha (phospho S32 + S36); p-IKB alpha (phosph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wu, Zhenhui et al. Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells Int J Mol Med, 42, 3157-3170 (2018)

PubMed: 30272289

Applications

IHC

Reactivity

Human

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

Immunohistochemical analysis of formalin-fixed and paraffin embedded human lung carcinoma tissue (dilution at:1:200) using p-IKB alpha antibody