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Orai1 Antibody

Catalog Number: orb570345

DispatchUsually dispatched within 5-10 working days
$ 268.00
Catalog Numberorb570345
CategoryAntibodies
DescriptionOrai1 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human Orai1 recombinant protein (Position: A49-A301).
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Dilution rangeWestern blot, 0.25-0.5μg/ml Flow Cytometry, 1-3μg/1x106 cells Direct ELISA, 0.1-0.5μg/ml
Form/AppearanceLyophilized
ConjugationUnconjugated
MW39 kDa
UniProt IDQ96D31
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Alternative namesCalcium release-activated calcium channel protein
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NoteFor research use only
Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Expiration Date12 months from date of receipt.
Orai1 Antibody

Western blot analysis of ORAI1 using anti-ORAI1 antibody (orb570345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human Jurka whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human K562 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORAI1 antigen affinity purified polyclonal antibody (Catalog # orb570345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for ORAI1 at approximately 39KD. The expected band size for ORAI1 is at 33KD.

Orai1 Antibody

Flow Cytometry analysis of CACO-2 cells using anti-ORAI1 antibody (orb570345). Overlay histogram showing CACO-2 cells stained with orb570345 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORAI1 Antibody (orb570345, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Orai1 Antibody

Flow Cytometry analysis of A549 cells using anti-ORAI1 antibody (orb570345). Overlay histogram showing A549 cells stained with orb570345 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORAI1 Antibody (orb570345, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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