OnePCR HiFi is a ready-to-use PCR reaction mixture. Simply adding primers and template, the reagent will execute primer extensions and other molecular biology applications. OnePCR HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly detected on the blue-light transilluminator or UV epi-illuminator after the DNA electrophoresis. The OnePCR HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of the primer and template solutions.