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Product Overview
Product Name One-Step RT-PCR System
Catalog Number orb180492
Hazard Information Non-Toxic
Target One-Step RT-PCR System
Product Properties
Storage Store at-20°C
Note For research use only.
Product Description

The One-Step RT-PCR with HotStar Taq System is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps.

Application Notes
Application Notes The kit consists of two major components: RT/ HotStar Taq Mix and 2X Reaction Mix. The RT/ HotStar Taq Mix contains a mixture of Reverse Transcriptase and HotStar Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. Reverse transcriptase is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability. HotStar Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C. This provides an automatic hot start in PCR, increasing sensitivity, specificity, and yield.
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