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Product Overview
Product Name Occludin antibody
Catalog Number orb11181
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Bovine, Canine, Equine, Porcine
ConjugationUnconjugated
Tested applicationsELISA, Flow-Cyt, WB
Immunogen KLH conjugated synthetic peptide derived between 441-512 amino acids of human Occludin
Target Occludin
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 57 kDa
Uniprot ID Q16625 (Human); Q61146 (Mouse); Q6P6T5 (Rat)
NCBI NP_001192183.11; NM_001205254.11
Entrez 100506658
Product Description

Rabbit polyclonal antibody to Occludin, which is also known as OCLN. It is frequently found in the plasma membrane and bicellular tight junctions. It is expressed in the tight junctions of the epithelial and endothelial cells and is overexpressed in the kidney. This protein plays an extensive role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier and can also induce adhesion when expressed in cells lacking tight junctions. Along with this, occludin helps in the regulation of bicellular tight junction assembly and protein complex assembly.

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, Flow-Cyt: 3
Validation Images
Immunohistochemical staining of paraffin embedded rat eyeball tissue using Occludin antibody
Immunohistochemical staining of paraffin embedded rat eyeball tissue using Occludin antibody
Western blot of rat liver tissue lysates using Occludin antibody
Western blot of rat liver tissue lysates using Occludin antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded mouse embryo tissue using Occludin antibody
Immunohistochemical analysis of formalin fixed and paraffin embedded mouse embryo tissue using Occludin antibody
IHC-P of mouse kidney tissue (Occludin antibody at 1:300)
IHC-P of mouse kidney tissue (Occludin antibody at 1:300)
Western blot analysis of mouse kidney lysate using Occludin antibody
Western blot analysis of mouse kidney lysate using Occludin antibody
Western blot analysis of A549 Cell Lysate using Occludin antibody
Western blot analysis of A549 Cell Lysate using Occludin antibody
Flow cytometric analysis of 293FT cells using Occludin antibody
Flow cytometric analysis of 293FT cells using Occludin antibody
Western blot analysis of A549 Cell Lysate using Occludin antibody
Western blot analysis of A549 Cell Lysate using Occludin antibody
Additional Documents
References

Woolf, E. C. et al. The ketogenic diet for the treatment of malignant glioma. J. Lipid Res. 56, 10-May (2015)

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