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Catalog Number | orb220640 |
---|---|
Category | Tools |
Description | Detect mature miRNA oar-mir-118 expression normalized by U6 snRNA. |
Tested applications | PCR |
Target | oar-mir-118 |
Sensitivity | 10 copies of a standard mimics and as little as 25 pg of total RNA |
Storage | Store at 4°C for short term and at -20°C for long term storage |
Note | For research use only |
Application notes | 50 reactions for Real-Time PCR Experiment Notes: The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. |
Expiration Date | 12 months from date of receipt. |
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