NSFL1C Antibody

• Catalog Number: orb1256854
• Category: Antibodies
• Description: NSFL1C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 41kDa
• Target: NSFL1C
• UniProt ID: Q9UNZ2
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: P47, UBX1, UBXD1UBXN2C, dJ776F14.1, NSFL1 cofactor
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using NSFL1C antibody (orb1256854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using NSFL1C antibody (orb1256854). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug NSFL1C antibody (orb1256854). Western blot was performed from the immunoprecipitate using NSFL1C antibody (orb1256854) at a dilution of 1:1000.