NSFL1C Antibody

SKU: orb1256854

Description

NSFL1C Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
• Target: NSFL1C
• Molecular Weight: Observed: 41kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

P47, UBX1, UBXD1UBXN2C, dJ776F14.1, NSFL1 cofactor p47, NSFL1 (p97) cofactor (p47), SHP1 homolog, UBX domain-containing protein 2C, p97 cofactor p47

Western blot analysis of extracts of various cell lines, using NSFL1C antibody (orb1256854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using NSFL1C antibody (orb1256854). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of A-549 cells, using 3 ug NSFL1C antibody (orb1256854). Western blot was performed from the immunoprecipitate using NSFL1C antibody (orb1256854) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

NSFL1C

UniProt

Q9UNZ2