NODAL monoclonal antibody (M03), clone 5C3

• Catalog Number: orb2292507
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant NODAL.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5C3
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1 Kappa
• Immunogen: NODAL (NP_060525, 275 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGC
• NCBI: NP_060525
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged NODAL is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]

NODAL monoclonal antibody (M03), clone 5C3 Western Blot analysis of NODAL expression in HeLa.

NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in PC-12.

NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in Raw 264.7.

Western Blot detection against Immunogen (33.66 KDa).