NHE3 antibody

• Catalog Number: orb152657
• Category: Antibodies
• Description: Rabbit polyclonal to NHE3 (ATTO-488). Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:25)
• Conjugation: ATTO-488
• MW: 84kDa
• Target: NHE3
• Entrez: 24784
• UniProt ID: P26433
• NCBI: NP_036786
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: Sodium / Hydrogen Exchanger 3 antibody, SLC9A3 ant
• Note: For research use only
• Application notes: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.