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NFIA Antibody

Catalog Number: orb381082

DispatchUsually dispatched within 5-10 working days
$ 520.00
Catalog Numberorb381082
CategoryAntibodies
DescriptionNFIA Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IHC, WB
Predicted ReactivityBovine, Canine, Equine, Gallus, Hamster, Monkey, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human NFIA (180-224aa AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSG VFS), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Dilution rangeImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, MouseImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
Form/AppearanceLyophilized
ConjugationUnconjugated
MW55944 MW
UniProt IDQ12857
StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Alternative namesNuclear factor 1 A-type;NF1-A;Nuclear factor 1/A;C
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NoteFor research use only
Application notesTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Expiration Date12 months from date of receipt.
NFIA Antibody

Flow Cytometry analysis of K562 cells using anti-NFIA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

NFIA Antibody

Flow Cytometry analysis of SiHa cells using anti-NFIA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

NFIA Antibody

WB analysis of NFIA using anti-NFIA antibody.Lane 1:JURKAT cell;2:COLO320 cell.

NFIA Antibody

WB analysis of NFIA using anti-NFIA antibody.Lane 1:mouse HEPA1-6 cell;2:mouse SP2/0 cell.

NFIA Antibody

IHC analysis of NFIA using anti-NFIA antibody.NFIA was detected in paraffin-embedded section of mouse liver tissues.

NFIA Antibody

IHC analysis of NFIA using anti-NFIA antibody.NFIA was detected in paraffin-embedded section of rat liver tissues.

NFIA Antibody

IHC analysis of NFIA using anti-NFIA antibody.NFIA was detected in paraffin-embedded section of human mammary cancer tissues.

NFIA Antibody

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