NF-Y antibody

• Catalog Number: orb344587
• Category: Antibodies
• Description: NF-Y antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, Gel Supershift Assays, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 2.0 to 4.0 µl, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
• Conjugation: Unconjugated
• UniProt ID: P25208
• NCBI: P25208.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-NF-Y Antibody, Nuclear transcription f
• Note: For research use only
• Application notes: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific). Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 µl per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#611-1302) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate (#ABTS-100). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.
• Expiration Date: 12 months from date of receipt.