| Category | Primary antibodies |
|---|---|
| Catalog Number | orb98403 |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Tested applications | IHC-Fr, IHC-P, WB |
| Reactivity | Bovine, Canine, Gallus, Guinea pig, Hamster, Human, Monkey, Rabbit, Rat, Sheep |
| Clone ID | RNF402 |
| Isotype | IgM |
| Concentration | 1 mg/ml |
| Form/Appearance | Each vial contains 0.1 ml monoclonal antibody in PBS containing 0.09% sodium azide. The antibody is purified by ammonium sulphate precipitation and will threfore contains fetal calf serum derived protein contaminants |
| Conjugation | Unconjugated |
| UniProt ID | P12036 |
| Application notes | RNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:10-1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25-1:250 for immunoblotting appliCations. |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Description | Mouse monoclonal to Neurofilament |
| Expiration Date | 12 months from date of receipt. |
| Note | For research use only. |
| Source | RNF402 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a neurofilament preparation of calf brain tissue. |
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