Neurofilament antibody

Catalog Number: orb98403

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CategoryPrimary antibodies
Catalog Numberorb98403
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIHC-Fr, IHC-P, WB
ReactivityBovine, Canine, Gallus, Guinea pig, Hamster, Human, Monkey, Rabbit, Rat, Sheep
Clone IDRNF402
IsotypeIgM
Concentration1 mg/ml
Form/AppearanceEach vial contains 0.1 ml monoclonal antibody in PBS containing 0.09% sodium azide. The antibody is purified by ammonium sulphate precipitation and will threfore contains fetal calf serum derived protein contaminants
ConjugationUnconjugated
UniProt IDP12036
Application notesRNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:10-1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25-1:250 for immunoblotting appliCations.
StorageThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
DescriptionMouse monoclonal to Neurofilament
Expiration Date12 months from date of receipt.
NoteFor research use only.
SourceRNF402 is a Mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a neurofilament preparation of calf brain tissue.
  1. 3 images
    • Immunohistochemical staining of paraffin embedded human brain tissue using INA antibody (primary antibody dilution at: 1:10-50)
    • Western blot analysis of mouse brain tissue lysates (35ug/lane)using INA antibody (primary antibody dilution at: 1:100-250)
    INA antibody [orb97678]

    ICC, IF, IHC-P, WB

    Mouse

    Monoclonal

    Unconjugated

    400 μl
  2. 2 images
    • Immunohistochemical staining of human Cerebellum tissue using NEFH antibody
    • Immunohistochemical staining of rat Cerebellum tissue using NEFH antibody
    NEFH antibody [orb388517]

    FC, IHC-P, WB

    Bovine, Feline, Gallus, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Rodent

    Mouse

    Monoclonal

    100 μg, 20 μg
  3. 2 images
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using NEFM antibody
    • Western blot analysis of Mouse Brain lysate using NEFM antibody
    NEFM antibody [orb195429]

    ELISA, IHC-P, WB

    Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat

    Goat

    Polyclonal

    Unconjugated

    50 μg
  4. 2 images
    • Immunohistochemical staining of human brain tissue using NEFH antibody
    • Western blot analysis of mouse brain (Lane1) whole cell lysates using NEFH antibody
    NEFH antibody [orb412733] Custom conjugation service available

    ICC, IF, IHC-P, IP, WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    50 μl, 100 μl, 200 μl
  5. 2 images
    • Immunofluorescence analysis of rat spinal cord tissue using Neurofilament H antibody
    • Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue using Neurofilament H antibody
    Neurofilament H antibody [orb11117] Custom conjugation service available

    ELISA, FC, IF, WB, IHC

    Human, Mouse

    100 μl, 200 μl, 50 μl